Transforming Rhodamine Dyes for (d)STORM Super‐Resolution Microscopy via 1,3‐Disubstituted Imidazolium Substitution
Bowen Wang, Michael Xiong, Josephine Susanto, Xue Li, Wai‐Yee Leung, Ke Xu
Abstract
We introduce a strategy to optimize the photoswitching behavior of rhodamines for (d)STORM super-resolution microscopy. By replacing the benzene ring in the rhodamine core with a permanently charged 1,3-disubstituted imidazolium, the resultant dyes are markedly sensitized toward photoswitching, and exhibit outstanding (d)STORM performance with fast on-off switching, long-lasting blinking, and bright single-molecule emission. We thus attain excellent (d)STORM images under green excitation that are on par with the "ideal" red-excited dyes, including for difficult structures such as the mammalian actin cytoskeleton, and demonstrate high-quality two-color three-dimensional (d)STORM.