A genome‐wide association study identifies a transporter for zinc uploading to maize kernels
Zhen‐Fei Chao, Yuanyuan Chen, Ji Chen, Yaling Wang, Xing Huang, Chu‐Ying Zhang, Jun Yang, Tao Song, Jiachen Wu, Liangxing Guo, Chu‐Bin Liu, Mei‐Ling Han, Yongrui Wu, Jianbing Yan, Dai‐Yin Chao
Abstract
The Zn content in cereal seeds is an important trait for crop production as well as for human health. However, little is known about how Zn is loaded to plant seeds. Here, through a genome-wide association study (GWAS), we identify the Zn-NA (nicotianamine) transporter gene ZmYSL2 that is responsible for loading Zn to maize kernels. High promoter sequence variation in ZmYSL2 most likely drives the natural variation in Zn concentrations in maize kernels. ZmYSL2 is specifically localized on the plasma membrane facing the maternal tissue of the basal endosperm transfer cell layer (BETL) and functions in loading Zn-NA into the BETL. Overexpression of ZmYSL2 increases the Zn concentration in the kernels by 31.6%, which achieves the goal of Zn biofortification of maize. These findings resolve the mystery underlying the loading of Zn into plant seeds, providing an efficient strategy for breeding or engineering maize varieties with enriched Zn nutrition.