Plasmonic heating-based portable digital PCR system
Christian D. Ahrberg, Ji Wook Choi, Jong Min Lee, Kyoung G. Lee, Seok Jae Lee, A. Manz, Bong Geun Chung
Abstract
. Target DNA concentrations in the range from 101 ± 1.4 copies per μL to 260 000 ± 20 000 copies per μL could be detected using a poly(dimethylsiloxane) (PDMS) microwell membrane with 22 080 well arrays (20 μm diameter). Furthermore, the heating system was demonstrated using a mass producible poly(methyl methacrylate) PMMA microwell array with 8100 microwell arrays (80 μm diameter). The PMMA microwell array could detect a concentration from 12 ± 0.7 copies per μL to 25 889 ± 737 copies per μL.
Topics & Concepts
Digital polymerase chain reactionPlasmonComputer scienceComputational biologyNanotechnologyEngineeringMaterials scienceBiologyOptoelectronicsPolymerase chain reactionGeneticsGeneBacteriophages and microbial interactionsBiosensors and Analytical DetectionMolecular Biology Techniques and Applications