Reshaping the binding pocket of purine nucleoside phosphorylase for improved production of 2-halogenated-2′-deoxyadenosines
Gaofei Liu, Xin Tong, Jialing Wang, Bin Wu, Jianlin Chu, Yongchan Jian, Bingfang He
Abstract
Semi-rational design and iterative combinatorial mutation of <italic>Am</italic>PNP with gratifyingly improved activity toward steric impediment of 2-halogenated-2′-deoxyadenosine biosynthesis.
Topics & Concepts
DeoxyadenosinePurine nucleoside phosphorylaseChemistrySteric effectsPurineStereochemistryCombinatorial chemistryRational designBiochemistryEnzymeBiologyGeneticsBiochemical and Molecular ResearchAdenosine and Purinergic SignalingCytomegalovirus and herpesvirus research