A cationic lipid with advanced membrane fusion performance for pDNA and mRNA delivery
Yu Wei, Ting He, Qunjie Bi, Huan Yang, Xueyi Hu, Rongrong Jin, Hong Liang, Yongqun Zhu, Rongsheng Tong, Yu Nie
Abstract
= 0, 1) of hydrophobic tails. Notably, all self-assemblies with nucleic acid have significant differences in particle size, stability in serum, membrane fusion, and fluidity. Moreover, the novel mRNA/pDNA formulations are characterized by overall low cytotoxicity, efficient compaction, protection, and release of nucleic acids. We find that the length of hydrophobic tails dominates the formation and stability of the assembly. And at a certain length, the unsaturated hydrophobic tails enhance the membrane fusion and fluidity of assemblies and thus significantly affect the transgene expression, followed by the number of hydrophobic tails.