Cross-Linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics
Christian Ihling, Lolita Piersimoni, Marc Kipping, Andrea Sinz
Abstract
ribosome, and 1623 unique cross-links for nuclear lysates, corresponding to 1088 intra- and 535 interprotein interactions and yielding 564 distinct protein-protein interactions. Our results underline the strength of combining XL-MS with ion mobility not only for deriving three-dimensional (3D) structures of single proteins but also for performing system-wide protein interaction studies.
Topics & Concepts
ChemistryMass spectrometryIon-mobility spectrometryFragmentation (computing)IonBovine serum albuminProtein–protein interactionEscherichia coliTandem mass spectrometryBiophysicsChromatographyBiochemistryOrganic chemistryOperating systemBiologyComputer scienceGeneMass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsProtein Structure and Dynamics