Secondary Metabolites Produced by Coculture of <i>Pleurotus ostreatus</i> SY10 and <i>Pleurotus eryngii</i> SY302
Xiaoxuan Ge, Yu Wang, Chunxiao Sun, Ziping Zhang, Lin Song, Lingling Tan, Dehai Li, Song Yang, Guihong Yu
Abstract
Abstract A new isovaleryl‐monoterpene, pleurotusin A ( 1 ), and a new cyclopentenone derivative, pleurotusin B ( 7 ), together with five related known terpenoids ( 2 – 6 ), were isolated from the coculture broth of two edible fungi, Pleurotus ostreatus SY10 and Pleurotus eryngii SY302. The absolute configurations of 1 and 7 were elucidated by comprehensively using the density functional theory calculation of NMR and ECD data, DP4+ probability analysis, Mo 2 (OAc) 4 ‐based CD experiment and optical rotation. The antimicrobial activities of these compounds except for the unstable compound 7 , were evaluated against two types of human‐pathogenic fungi, Candida albicans and Cryptococcus neoformans , and three types of human‐pathogenic bacteria, Staphylococcus aureus , Escherichia coli , and Shigella sp. Compound 1 displayed moderate activity against S. aureus with an MIC 50 value of 90.3 μM. In addition, the antioxidant activities of high‐yielding 2 – 6 were tested using DPPH, and compound 4 showed moderate activity with an EC 50 value of 573 μM.