Light field microscopy in biological imaging
Chengqiang Yi, Lanxin Zhu, Dongyu Li, Peng Fei
Abstract
Light field microscopy (LFM), featured for high three-dimensional imaging speed and low phototoxicity, has emerged as a technique of choice for instantaneous volumetric imaging. In contrast with other scanning-based three-dimensional (3D) imaging approaches, LFM enables to encode 3D spatial information in a snapshot manner, permitting high-speed 3D imaging that is only limited by the frame rate of the camera. In this review, we first introduce the fundamental theory of LFM and current corresponding advanced approaches. Then, we summarize various applications of LFM in biological imaging.
Topics & Concepts
Snapshot (computer storage)Frame rateMicroscopyOpticsHyperspectral imagingComputer scienceLight sheet fluorescence microscopySpectral imagingLight fieldComputer visionArtificial intelligencePhysicsScanning confocal electron microscopyOperating systemAdvanced Fluorescence Microscopy TechniquesDigital Holography and MicroscopyOptical Coherence Tomography Applications