Lost and Found: Re-searching and Re-scoring Proteomics Data Aids Genome Annotation and Improves Proteome Coverage
Patrick J. Willems, Igor Fijałkowski, Petra Van Damme
Abstract
Delineation of open reading frames (ORFs) causes persistent inconsistencies in prokaryote genome annotation. We demonstrate that by advanced (re)analysis of omics data, a higher proteome coverage and sensitive detection of unannotated ORFs can be achieved, which can be exploited for conditional bacterial genome (re)annotation, which is especially relevant in view of annotating the wealth of sequenced prokaryotic genomes obtained in recent years.
Topics & Concepts
ORFSProteomeGenomeComputational biologyAnnotationBiologyProteogenomicsGenome projectBacterial genome sizeHuman proteome projectProteomicsGene AnnotationGeneticsComputer scienceGeneGenomicsOpen reading framePeptide sequenceGenomics and Phylogenetic StudiesRNA and protein synthesis mechanismsIdentification and Quantification in Food