Optical nanoscopy
Alberto Diaspro, Paolo Bianchini
Abstract
Abstract This article deals with the developments of optical microscopy towards nanoscopy. Basic concepts of the methods implemented to obtain spatial super-resolution are described, along with concepts related to the study of biological systems at the molecular level. Fluorescence as a mechanism of contrast and spatial resolution will be the starting point to developing a multi-messenger optical microscope tunable down to the nanoscale in living systems. Moreover, the integration of optical nanoscopy with scanning probe microscopy and the charming possibility of using artificial intelligence approaches will be shortly outlined.
Topics & Concepts
Nanoscopic scaleMicroscopyResolution (logic)Optical microscopeSuper-resolution microscopyImage resolutionOpticsSuperresolutionNanotechnologyComputer scienceMaterials sciencePhysicsArtificial intelligenceScanning confocal electron microscopyImage (mathematics)Scanning electron microscopeAdvanced Fluorescence Microscopy TechniquesNear-Field Optical MicroscopyForce Microscopy Techniques and Applications