Biogenic Palladium Improved Perchlorate Reduction during Nitrate Co-Reduction by Diverting Electron Flow in a Hydrogenotrophic Biofilm
Jingzhou Zhou, Lin Yang, Xiaodi Li, Ben Dai, Junxia He, Chengyang Wu, Si Pang, Siqing Xia, Bruce E. Rittmann
Abstract
Microbial reduction of perchlorate (ClO 4 – ) is emerging as a cost-effective strategy for groundwater remediation. However, the effectiveness of perchlorate reduction can be suppressed by the common co-contamination of nitrate (NO 3 – ). We propose a means to overcome the limitation of ClO 4 – reduction: depositing palladium nanoparticles (Pd 0 NPs) within the matrix of a hydrogenotrophic biofilm. Two H 2 -based membrane biofilm reactors (MBfRs) were operated in parallel in long-term continuous and batch modes: one system had only a biofilm (bio-MBfR), while the other incorporated biogenic Pd 0 NPs in the biofilm matrix (bioPd-MBfR). For long-term co-reduction, bioPd-MBfR had a distinct advantage of oxyanion reduction fluxes, and it particularly alleviated the competitive advantage of NO 3 – reduction over ClO 4 – reduction. Batch tests also demonstrated that bioPd-MBfR gave more rapid reduction rates for ClO 4 – and ClO 3 – compared to those of bio-MBfR. Both biofilm communities were dominated by bacteria known to be perchlorate and nitrate reducers. Functional-gene abundances reflecting the intracellular electron flow from H 2 to NADH to the reductases were supplanted by extracellular electron flow with the addition of Pd 0 NPs.