<i>bsh</i>1 Gene of <i>Lactobacillus plantarum</i> AR113 Plays an Important Role in Ameliorating Western Diet-Aggravated Colitis
Junlin Shao, Zhiyong Mu, Yongjun Xia, Zhiqiang Xiong, Xin Song, Yijin Yang, Hui Zhang, Lianzhong Ai, Guangqiang Wang
Abstract
Western diet is thought to increase susceptibility to inflammatory bowel disease (IBD), and probiotics are a potential therapeutic agent for IBD. This study revealed the effects of Lactobacillus plantarum AR113 and L. plantarum AR113Δ bsh 1 on a dextran sulfate sodium (DSS)-induced colitis mouse model under the Western diet (WD). After four weeks of WD and low-sugar and low-fat diet (LD) intervention, induction with 3% DSS, and intragastric administration of probiotics, we found that L. plantarum AR113 could regulate blood glucose and lipid levels and have a certain protective effect on hepatocytes. Our results suggested that the L. plantarum AR113 alleviated DSS-induced colitis under the Western diet by improving dyslipidemia, repairing intestinal barrier dysfunction, and inhibiting the TLR4/Myd88/TRAF-6/NF-κB inflammatory pathway. However, these changes were not demonstrated in the L. plantarum AR113Δ bsh 1, and therefore, we reasoned that the presence of bsh 1 may play a crucial role in the L. plantarum AR113 exerting its anti-inflammatory function. The relationship between bile salt hydrolase (BSH) and colitis was worthy of further exploration.