Removal of AMR plasmids using a mobile, broad host-range CRISPR-Cas9 delivery tool
David Sünderhauf, Uli Klümper, Elizabeth Pursey, Edze R. Westra, William H. Gaze, Stineke van Houte
Abstract
. This study firmly establishes pKJK5::csg as a promising broad host-range CRISPR-Cas9 delivery tool for AMR plasmid removal, which has the potential to be applied in complex microbial communities to remove AMR genes from a broad range of bacterial species.
Topics & Concepts
PlasmidCRISPRCas9BiologyHost (biology)Escherichia coliHorizontal gene transferMobile genetic elementsGeneBacteriaMicrobiologyGeneticsGenomeCRISPR and Genetic EngineeringVibrio bacteria research studiesAntibiotic Resistance in Bacteria