Directed evolution of <i>Rhodotorula gracilis</i><scp>d</scp>-amino acid oxidase using single-cell hydrogel encapsulation and ultrahigh-throughput screening
Christoph Küng, Rosario Vanella, Michael A. Nash
Abstract
A high-throughput screening platform uses peroxidase-mediated proximity labeling with fluorescent hydrogels. A yeast displayed enzyme variant library was screened using a reaction cascade. Enhancement of enzyme K m is observed for displayed enzymes.
Topics & Concepts
Directed evolutionChemistryEncapsulation (networking)YeastCell encapsulationCellBiochemistryComputer scienceGeneMutantComputer networkAmino Acid Enzymes and MetabolismPolyamine Metabolism and ApplicationsMass Spectrometry Techniques and Applications