Use of tandem affinity–buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins
Stella M. Lai, Pankajavalli Thirugnanasambantham, Vaishnavi Sidharthan, Andrew Norris, Jamison D. Law, Venkat Gopalan, Vicki H. Wysocki
Topics & Concepts
ChemistryChromatographyRecombinant DNAMass spectrometryAffinity chromatographyProtein purificationElutionHeterologousTandem affinity purificationGel electrophoresisMolecular massTandem mass spectrometryDenaturation (fissile materials)BiochemistryEnzymeGeneNuclear chemistryProtein purification and stabilityMonoclonal and Polyclonal Antibodies ResearchProtein Structure and Dynamics