Optimizing The Cell Seeding Protocol to Human Decellularized Ovarian Scaffold: Application of Dynamic System for Bio-Engineering.
Leila Mirzaeian, Farideh Eivazkhani, Maryam Hezavehei, Ashraf Moini, Fereshteh Esfandiari, Mojtaba Rezazadeh Valojerdi, Rouhollah Fathi
Abstract
OBJECTIVE: functions of the ovary. On the other hand, the scaffold needs interaction and integration with cells. Thus, the development of ovarian engineered constructs (OVECs) requires the use of efficient methods for seeding of the cells into the ovarian and other types of scaffolds. The main goal of the present study was to develop an optimized culture system for efficient seeding of peritoneum mesenchymal stem cells (PMSCs) into human decellularized ovarian scaffold. MATERIALS AND METHODS: In this experimental study, three methods were used for cellular seeding including rotational (spinner flask) and static (conventional and injection) seeding cultures. OVECs were evaluated with Hematoxylin and Eosin staining and viability analyses for the seeded PMSCs. Then, immunohistochemistry analysis was performed using the best method of cellular seeding for primordial germ cell-like cells, mesenchymal stem cells and proliferation markers. Stereology analysis was also performed for the number of penetrated cells into the OVECs. RESULT: on each OVEC. CONCLUSION: The rotational seeding method is a more favorable approach to cell seeding into ovarian decellularized tissue than static seeding.