Preparation, Antibacterial and Antiviral Activity Measurements and Detection Methods
Amol D. Gholap, Sagar R. Pardeshi, Prabhanjan Giram
Abstract
In order to tackle resistance, there has been a rise in curiosity in investigating and creating new antimicrobial and antiviral medicines from different sources. As a result, approaches for testing and assessing antimicrobial and antiviral activity have received more attention. Some bioassays, like disk-diffusion, well diffusion, and broth or agar dilution, are well recognized and frequently utilized assays for microbes, but others, like flow cytofluorometric and bioluminescent methods, are less popular since they need specialized equipment and additional testing for reproducibility and standardization. In-depth descriptions have also been provided of developments in molecular biology, computer technology, and knowledge of the molecular structure of viruses. This data is a first step in determining the effectiveness of new antiviral drugs based on techniques evaluating suppression of the intended target. Here, we offer a summary of assays measuring the effectiveness of inhibitors of clinically significant bacteria and viruses using biochemical and cell-based methods.