Evidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex
Laura Rank, Laura E. Herring, Miriam Braunstein
Abstract
How lipids travel between membranes of diderm bacteria is a challenging mechanistic question because lipids, which are hydrophobic molecules, must traverse a hydrophilic periplasm. This question is even more complex for mycobacteria, which have a unique cell envelope that is highly impermeable to molecules. A growing body of knowledge identifies Mce transporters as lipid importers for mycobacteria. Here, using protein stability experiments and immunoprecipitation-mass spectrometry, we provide evidence for mycobacterial Mce transporters existing as multiprotein complexes.
Topics & Concepts
Periplasmic spaceBiologyMultiprotein complexCell envelopeBacteriaMembraneBacterial outer membraneBiochemistryCell membraneCell biologyBiophysicsComputational biologyEscherichia coliGeneticsGeneTuberculosis Research and EpidemiologyMycobacterium research and diagnosisAntibiotic Resistance in Bacteria