In-Cell Quantification of Drugs by Magic-Angle Spinning Dynamic Nuclear Polarization NMR
Andrea Bertarello, Pierrick Berruyer, Markus Artelsmair, Charles S. Elmore, Sepideh Heydarkhan‐Hagvall, Markus Schade, Elisabetta Chiarparin, Staffan Schantz, Lyndon Emsley
Abstract
N]CHIR-98014, an activator of the Wingless and Int-1 signaling pathway, determining intracellular drug amounts in the range of tens to hundreds of picomoles per million cells. This is, to our knowledge, the first time that MAS DNP has been used to successfully estimate intracellular drug amounts.
Topics & Concepts
IntracellularChemistryMagic angle spinningActivator (genetics)DrugBiophysicsNuclear magnetic resonance spectroscopyAnalytical Chemistry (journal)ChromatographyBiochemistryStereochemistryPharmacologyReceptorBiologyMedicineDNA and Nucleic Acid ChemistryAdvanced NMR Techniques and ApplicationsProtein Structure and Dynamics