A photoluminescence biosensor for the detection of <i>N</i>-acyl homoserine lactone using cysteamine functionalized ZnO nanoparticles for the early diagnosis of urinary tract infections
Sahana Vasudevan, Parthasarathy Srinivasan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Abstract
acceptor defect level of ZnO with reference to ZnO-Cys were considered. A maximum sensitivity of 97% was achieved in the detection of AHL. The linear detection range of the developed biosensor was 10-120 nM in artificial urine media (AUM). The effect of pH on the sensitivity of the biosensor in AUM was also investigated and reported. The developed sensor was validated using the AHLs produced by Pseudomonas aeruginosa (MCC3101) in real-time analysis, which further confirmed the overall specificity and sensitivity.
Topics & Concepts
PhotoluminescenceBiosensorCysteamineNanoparticleUrinary systemMaterials scienceHomoserineLactoneCombinatorial chemistryNanotechnologyQuorum sensingOrganic chemistryBiochemistryChemistryMedicineOptoelectronicsEndocrinologyVirulenceGeneMolecular Sensors and Ion DetectionNeonatal Health and BiochemistryBiomedical Research and Pathophysiology