Gold nanoclusters cause selective light-driven biochemical catalysis in living nano-biohybrid organisms
John R. Bertram, Yuchen Ding, Prashant Nagpal
Abstract
NC-nanorgs demonstrate 29.3% quantum efficiency of converting absorbed photons to the desired chemical, and 12.9% efficiency of photon-to-fuel conversion based on energy input-output. Further, by comparing the light-driven ammonia production yield between strains producing Mo-Fe nitrogenase with and without histidine tags, we demonstrate that preferential coupling of Au NCs to the nitrogenase through Au-histidine interactions is crucial for effective electron transfer and subsequent product generation. Together, these results provide the design rules for forming Au NCs-nanorgs and can have important implications for carrying out light-driven biochemical catalysis for renewable solar fuel generation.