Manipulating regioselectivity of an epoxide hydrolase for single enzymatic synthesis of (<i>R</i>)-1,2-diols from racemic epoxides
Die Hu, Xun-Cheng Zong, Feng Xue, Chuang Li, Bo-Chun Hu, Minchen Wu
Abstract
Both the activity and regioselectivity of Phaseolus vulgaris epoxide hydrolase were remarkably improved via reshaping two substrate tunnels based on rational design. The elegant one-step enantioconvergent hydrolysis of seven rac-epoxides was achieved by single mutants, allowing green and efficient access to valuable (R)-1,2 diols with high eep (90.1-98.3%) and yields.
Topics & Concepts
RegioselectivityEpoxide hydrolaseEpoxideChemistryEnzymeStereochemistryEpoxide hydrolase 2DiolBiocatalysisOrganic chemistryCombinatorial chemistryCatalysisReaction mechanismMicrosomeEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionBiochemical Acid Research Studies