Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants
Yuanyuan Zhang, Xiaoyan Zhang, Huan Cheng, Mario Roque Huanca Nina, Jun Ge, Yunpeng Bai
Abstract
Engineering of a promiscuous lactonase via semi-rational evolution gave a 1007-fold improvement in its catalytic activity in the degradation of triphenyl phosphate (TPHP). TPHP is a typical bulky organophosphate flame retardant (OPFR) and is widely used in industry. To the best of our knowledge, this is the first artificial enzyme capable of degrading OPFRs.
Topics & Concepts
OrganophosphateChemistryFire retardantOrganic chemistryPesticideBiologyAgronomyToxic Organic Pollutants ImpactFlame retardant materials and propertiesMicrobial bioremediation and biosurfactants