DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis
Jee‐Heun Kim, So‐Yeon Park, So-El Jeon, Jang-Hyun Choi, Choong-Jae Lee, Tae‐Young Jang, Hyeon-Ji Yun, Yuno Lee, Pilho Kim, Sang‐Hee Cho, Ji Shin Lee, Jeong‐Seok Nam
Abstract
Rationale: Doublecortin-like kinase 1 (DCLK1) is a serine/threonine kinase that selectively marks cancer stem-like cells (CSCs) and promotes malignant progression in colorectal cancer (CRC). However, the exact molecular mechanism by which DCLK1 drives the aggressive phenotype of cancer cells is incompletely determined. Methods: Here, we performed comprehensive genomics and proteomics analyses to identify binding proteins of DCLK1 and discovered X-ray repair cross-complementing 5 (XRCC5). Thus, we explored the biological role and downstream events of the DCLK1/XRCC5 axis in human CRC cells and CRC mouse models.