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Functional Dynamics of an Ancient Membrane-Bound Hydrogenase

Max E. Mühlbauer, Ana P. Gámiz‐Hernández, Ville R. I. Kaila

2021Journal of the American Chemical Society37 citationsDOIOpen Access PDF

Abstract

binding and protonation changes at a putative ion-binding site couple to proton transfer across the antiporter-like MbhH subunit by modulating the conformational state of a conserved ion pair at the subunit interface. Our findings illustrate conserved coupling principles within the complex I superfamily and provide functional insight into archaeal energy transduction mechanisms.

Topics & Concepts

ChemistryHydrogenaseAntiporterPyrococcus furiosusArchaeaProtonationAntiportersMolecular dynamicsBiophysicsProtein subunitBioenergeticsMembraneIonBiochemistryComputational chemistryEnzymeBiologyGeneMitochondrionOrganic chemistryMetalloenzymes and iron-sulfur proteinsHydrogen Storage and MaterialsPhotosynthetic Processes and Mechanisms
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