MiRNA-155 Biosensors Based on AlGaN/GaN Heterojunction Field Effect Transistors With an Au-SH-RNA Probe Gate
Yue He, Ke-Yue Chen, Tingting Wang, Mao Jia, Lihua Bai, Xiao Wang, Yuyu Bu, Jin‐Ping Ao
Abstract
AlGaN/gallium nitride (GaN) heterojunction field effect transistors (HFETs) have been researched widely in the fields of gas detection and ions detection. In this article, we develop a biosensor based on AlGaN/GaN HFETs to detect the tumor biomarker miRNA-155 directly. By fixing the specific sulfhydryl modified ribonucleic acid (SH-RNA) probe on the Au-gate surface of the AlGaN/GaN HFET to fabricate an RNA-Au-AlGaN/GaN HFET biosensor, this biosensor can conveniently and rapidly detect miRNA-155. The experimental results show that this biosensor has an excellent detection limit of 1.81 fM and a high sensitivity of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$77\,\mu \text{A}$ </tex-math></inline-formula> /log concentration with a linear range (2 fM–2 nM). The limit of detection is the lowest among the reported AlGaN/GaN HFET RNA biosensors. Meanwhile, this biosensor shows a high specific recognition capacity but is weak sensitive to the mismatched miRNA.