Quick Access to Nucleobase-Modified Phosphoramidites for the Synthesis of Oligoribonucleotides Containing Post-Transcriptional Modifications and Epitranscriptomic Marks
Kamil Ziemkiewicz, Marcin Warmiński, Radoslaw Wojcik, Joanna Kowalska, Jacek Jemielity
Abstract
G). Such modification of nucleobases is a common mechanism for post-transcriptional regulation of RNA stability and translational activity in various organisms. To better understand this process, relevant cellular recognition partners (e.g., proteins) must be identified and characterized. However, this step has been impeded by limited access to molecular tools containing such modified nucleotides.
Topics & Concepts
ChemistryNucleobaseNucleotideOligonucleotideNucleosideCombinatorial chemistryRNADeprotonationAlkylStereochemistryDNANucleophileElectrophileRNase HNucleic acidBiochemistryRNase POrganic chemistryGeneIonCatalysisRNA modifications and cancerRNA and protein synthesis mechanismsRNA Research and Splicing