Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors
Yuanhuan Yu, Xin Wu, Ningzi Guan, Jiawei Shao, Huiying Li, Yuxuan Chen, Ping Yuan, Dali Li, Haifeng Ye
Abstract
oncogene in a mouse xenograft tumor model. Beyond extending the spectrum of light energies in optogenetic toolbox for CRISPR-Cas9 technologies, this study demonstrates how FAST system can be deployed for programmable deep tissue gene editing in both biological and biomedical contexts toward high precision and spatial specificity.
Topics & Concepts
Genome editingCRISPRCas9Homology directed repairComputer scienceComputational biologyOptogeneticsBiologyGeneGeneticsDNA repairNeuroscienceNucleotide excision repairCRISPR and Genetic EngineeringRetinal Development and DisordersGenetics, Aging, and Longevity in Model Organisms