The elucidation of phospholipid bilayer–small molecule interactions using a combination of phospholipid nanodiscs and solution state NMR techniques
Georgina Townshend, Gary S. Thompson, Lisa J. White, Jennifer R. Hiscock, Jose L. Ortega‐Roldan
Abstract
Quantifying phospholipid bilayer-small molecule interactions is vital to the development of new drug candidates and/or medicinal therapies. However, obtaining these data remains problematic. Herein, we detail a phospholipid nanodisc assay which enables the elucidation of these interactions using conventional solution state NMR spectroscopy techniques.
Topics & Concepts
NanodiscPhospholipidBilayerLipid bilayerChemistryNuclear magnetic resonance spectroscopySmall moleculeMoleculeBiophysicsMembraneBiochemistryStereochemistryOrganic chemistryBiologyLipid Membrane Structure and BehaviorAdvanced NMR Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies