NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway
Zhenhua Chen, Qian‐Zhi Ni, Xiuping Zhang, Ning Ma, Jing-Kai Feng, Kang Wang, Jingjing Li, Dong Xie, Xingyuan Ma, Shuqun Cheng
Abstract
; downregulation of NET1 was shown to exhibit an opposite inhibitory effect. RNA-seq analysis and gene set enrichment analysis demonstrated that knockdown of NET1 significantly suppressed the level of Akt phosphorylation level and the expression of Akt downstream genes in HCC cells. Moreover, MK2206, a potent Akt inhibitor was shown to block the NET1-induced effects in HCC. Taken together, this study demonstrated that, through the Akt signaling pathway, NET1 plays an oncogenic role in HCC progression and metastasis. Hence, NET1 may potentially be used as a potential therapeutic target and prognostic marker of HCC.
Topics & Concepts
In vivoProtein kinase BIn vitroCancer researchMetastasisPI3K/AKT/mTOR pathwaySignal transductionCell biologyChemistryBiologyMedicineInternal medicineCancerBiochemistryGeneticsCancer Mechanisms and TherapyPI3K/AKT/mTOR signaling in cancerCancer, Hypoxia, and Metabolism