Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
Ellen M. Quardokus, Diane C. Saunders, Elizabeth McDonough, John W. Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian, Lisa Lowery, Elizabeth K. Neumann, Frida Björklund, Taruna V. Neelakantan, Joshua Croteau, Anne E. Wiblin, Jeremy Fisher, April J. Livengood, Karen Dowell, Jonathan C. Silverstein, Jeffrey M. Spraggins, Gloria Pryhuber, Gail Deutsch, Fiona Ginty, Garry P. Nolan, Simon Melov, Danny Jonigk, Michael A. Caldwell, Ioannis S. Vlachos, Werner Müller, Nils Gehlenborg, Brent R. Stockwell, Emma Lundberg, M Snyder, Ronald N. Germain, Jeannie M. Camarillo, Neil L. Kelleher, Katy Börner, Andrea J. Radtke
Abstract
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.