Derivation of induced pluripotent stem cells line (RCPCMi007-A-1) with inactivation of the beta-2-microglobulin gene by CRISPR/Cas9 genome editing
Margarita E. Bogomiakova, Elizaveta K. Sekretova, Artem V. Eremeev, L.D. Shuvalova, Pavel A. Bobrovsky, Elena Zerkalenkova, Olga S. Lebedeva, Maria A. Lagarkova
Abstract
The mismatch of HLA haplotypes between donor and recipient adversely affects the outcome of tissue transplantation. The B2M gene knockout (B2M-KO) disrupts the HLA I heterodimer formation; therefore, B2M-KO cells have reduced immunogenicity to allogeneic CD8+ T cells. Thus, the B2M-KO IPSCs and their derivatives can potentially solve a problem of the immunological compatibility in allogeneic transplantations. Using CRISPR/Cas9-mediated genome editing, we generated a human B2M-KO iPSC line (RCPCMi007-A-1). The RCPCMi007-A-1 iPSCs express pluripotency markers, have typical stem cell morphology, maintain normal karyotype, and the ability to differentiate into three germ layers.