Optimized Phosphorus Application Under Water Stress Enhances Photosynthesis, Physiological Traits, and Yield in Soybean During Flowering Stage
Qu Chen, Tangzhe Nie, Yang Li, Hao Li, Yubo Sun, Zezhou Wu, Yuxian Zhang, Mengxue Wang
Abstract
Phosphorus application is widely regarded as a key measure for improving crop resistance to drought. This study investigated the effect of appropriate phosphorus fertilization on photosynthesis, physiological traits, and yield under water stress during the soybean flowering stage and selected the drought-sensitive soybean variety “Sui Nong 26” as the pot experiment object under a completely randomized design. The experiment was designed with three irrigation lower limits, corresponding to 70%, 60%, and 50% of the field capacity (FC), referred to as T1, T2, and T3. Four phosphorus fertilizer applications were also included: 0, 40, 50, and 60 mg·kg (designated as P0, P1, P2, and P3), resulting in a total of 12 treatments. Photosynthetic parameters, antioxidant enzyme activities, membrane lipid peroxidation, osmotic adjustment substances, yield, and yield components were measured to assess the effects of phosphorus fertilization on drought resistance. Results showed that under water stress, moderate phosphorus application (P1 and P2) enhanced photosynthetic capacity, antioxidation, osmotic adjustment, and yield, particularly by scavenging excess reactive oxygen species, protecting cells from oxidative damage, and maintaining metabolic balance, leading to increased yield. The average net photosynthetic rate and yield per plant under P1 and P2 levels increased by 33.53% and 37.67%, and 20.7% and 15.6%, respectively, compared to P0. In contrast, excessive phosphorus application (P3) improved the above parameters but had a significantly lower effect than moderate phosphorus application. Thus, appropriate phosphorus application is crucial for soybeans under water stress. Moderate application not only alleviates drought stress but also boosts soybean yield. This study highlights the importance of appropriate phosphorus use for mitigating water stress, offering scientific evidence for its practical application in agriculture. At the same time, with the increasing severity of climate change and water scarcity, phosphorus fertilizer application strategies under varying water conditions provide critical support for the application of precision agriculture technologies and ensuring food security.