Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells
Thomas Bayer, Lara Pfaff, Yannick Branson, Aileen Becker, Shuke Wu, Uwe T. Bornscheuer, Ren Wei
Abstract
, which yielded bioluminescence that not only semiquantitatively reflected amounts of TPA in hydrolysis samples but is suitable as a high-throughput screening assay to assess PET hydrolase activity. Furthermore, the CAR-catalyzed synthesis of terephthalaldehyde was combined with a reductive amination cascade in a one-pot setup yielding the corresponding diamine, suggesting a new strategy for the transformation of TPA as a product obtained from PET biodegradation.
Topics & Concepts
Terephthalic acidPolyethylene terephthalatePolyesterReductive aminationHydrolysisPolylactic acidChemistryBiosensorCombinatorial chemistryBiodegradationEscherichia coliCatalysisOrganic chemistryBiochemistryPolymerMaterials scienceComposite materialGeneMicroplastics and Plastic PollutionEffects and risks of endocrine disrupting chemicalsbiodegradable polymer synthesis and properties