Mutation in the Plasmodium falciparum BTB/POZ Domain of K13 Protein Confers Artemisinin Resistance
Lucie Paloque, Romain Coppée, Barbara H. Stokes, Nina F. Gnädig, Karamoko Niaré, Jean-Michel Augereau, David A. Fidock, Jérôme Clain, Françoise Benoit-Vical
Abstract
Partial artemisinin resistance, defined in patients as a delayed parasite clearance following artemisinin-based treatment, is conferred by non-synonymous mutations in the Kelch beta-propeller domain of the Plasmodium falciparum k13 ( pfk13 ) gene. Here, we carried out in vitro selection over a 1-year period on a West African P. falciparum strain isolated from Kolle (Mali) under a dose-escalating artemisinin regimen.
Topics & Concepts
Plasmodium falciparumArtemisininBiologyDihydroartemisininGeneMutationSanger sequencingVirologyCoding regionPoint mutationGeneticsStrain (injury)Drug resistanceMolecular biologyParasite hostingSequence analysisGene mutationApicomplexaAtovaquoneMalaria Research and ControlCalpain Protease Function and RegulationMultiple Myeloma Research and Treatments