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PTM-Mamba: a PTM-aware protein language model with bidirectional gated Mamba blocks

Fred Zhangzhi Peng, Chentong Wang, Tong Chen, Benjamin Schussheim, Sophia Vincoff, Pranam Chatterjee

2025Nature Methods36 citationsDOIOpen Access PDF

Abstract

Current protein language models (LMs) accurately encode protein properties but have yet to represent post-translational modifications (PTMs), which are crucial for proteomic diversity and influence protein structure, function and interactions. To address this gap, we develop PTM-Mamba, a PTM-aware protein LM that integrates PTM tokens using bidirectional Mamba blocks fused with ESM-2 protein LM embeddings via a newly developed gating mechanism. PTM-Mamba uniquely models both wild-type and PTM sequences, enabling downstream tasks such as disease association and druggability prediction, PTM effect prediction on protein-protein interactions and zero-shot PTM discovery. In total, our work establishes PTM-Mamba as a foundational tool for PTM-aware protein modeling and design.

Topics & Concepts

DruggabilityComputer scienceComputational biologyENCODEFunction (biology)BiologyGeneticsGeneMachine Learning in BioinformaticsRNA and protein synthesis mechanismsGenomics and Phylogenetic Studies
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