Human epidermal stem cell differentiation is modulated by specific lipid subspecies
Matteo Vietri Rudan, Ajay Mishra, Christian Klose, Ulrike Eggert, Fiona M. Watt
Abstract
Significance Cells generate a vast repertoire of lipid molecules whose functions are poorly understood. To investigate whether lipids can regulate cell fate decisions, we carried out a systematic lipidomic analysis and perturbation of lipid metabolism in cultured human epidermal keratinocytes, determining associations with the onset of differentiation. We identified individual lipid species that induced exit from the epidermal stem cell compartment. Our observations suggest that more research is warranted on the regulation of biological processes via lipid species, moving beyond the more conventional contribution of proteins and nucleic acids.
Topics & Concepts
Cell biologyBiologyCellular differentiationLipid dropletEpidermis (zoology)Gene knockdownKeratinocyteLipid metabolismStem cellBiochemistryGeneIn vitroAnatomyLipid Membrane Structure and BehaviorLipid metabolism and biosynthesisRNA Interference and Gene Delivery