Eco-Friendly Route for Encapsulation of 5-Fluorouracil Into Polycaprolactone Nanoparticles
Moshera Samy, Heba M. Abdallah, Magdy M. H. Ayoub, М. Э. Вылегжанина, A. Ya. Volkov, T. E. Sukhanova
Abstract
The main objective of this study was to allocate and evaluate the most optimum conditions to prepare the 5-Fluorouracil (5-FU) entrapped polycaprolactone (PCL) nanoparticles (5-FU–PCNs) by eco-friendly route. The inner aqueous phase (W1) was added to solution containing PCL with homogenization to form primary emulsion (W1/O) which was emulsified with the outer aqueous phase (W2) containing polyvinyl alcohol (PVA) as stabilizer to attain the double emulsion (W1/O/W2). The different parameters were investigated to reach the most successful formulation for 5-FU–PCNs, such as polymer concentration, effect of stabilizer concentration ratio (0.5%, 1% and 3%) on drug encapsulation efficiency, etc. The encapsulation efficiency of PCNs was in the range of 14-65.6%. The prepared nanoparticles showed the spherical shape having an average size of 183-944nm.The prepared systems were elucidated by fourier transform infrared spectroscopy (FTIR), UV-spectroscopy, photon correlation spectroscopy (PCS), X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA),scanning electron (SEM) and atomic-force microscopy (AFM). The optimized ratios of the different parameters of double emulsion process were founded. SEM and AFM confirmed the complicated morphology and the formation of the spherical micro- and nanocapsules.