Purification, Characterization and Protective Effects of Indigenous Yeast Derived β-glucans against Salmonellosis in Broilers
Unknown authors
Abstract
Indigenous Saccharomyces (S.) cerevisiae was cultured under submerged fermentation conditions and β-glucans were extracted by enzymatic method.The extracted β-glucans were characterized by FT-IR and their spectra presented peaks at a wavelength of 1021 cm -1 .Safety and mutagenicity testing of β-glucans showed them non-mutagenic and non-toxic up to a concentration of 200 mg/mL.For immunotherapeutic effects against Salmonella (Sal.)typhimurium infection, broiler chicks (n=120) were randomly divided into 4 equal groups viz.A (negative control), B (positive control challenged with Sal.typhimurium; 1.0× 10 10 cfu), C (β-glucans at the dose rate of 50 mg/kg feed + Sal.typhimurium challenge), and D (β-glucans at the dose rate of 100 mg/kg feed + Sal.typhimurium challenge).Results revealed protective effects of β-glucans against Sal.typhimurium infection as depicted by mild clinical signs along with minimal/no gross and histopathological lesions.Immunological responses were also higher (P<0.05) in β-glucans administered birds.β-glucans also modulated intestinal morphology with improved villi-height and crypt-depth ratio.In conclusion, β-glucans isolated from indigenous S. cerevisiae conferred protective effects against salmonellosis and therefore can be used successfully in broilers.