A Surface-Enhanced Raman Scattering-Based Probe Method for Detecting Chromogranin A in Adrenal Tumors
Xiaoyu Cui, Dayu Hu, Chengyuan Wang, Shuo Chen, Zeyin Zhao, Xiaosong Xu, Yudong Yao, Tao Liu
Abstract
Aim: We aim to demonstrate that a surface-enhanced Raman spectroscopy (SERS) probe can be effectively used for protein detection in adrenal tumors. Materials & methods: The SERS probe method, which uses Au@Ag core-shell nanoparticles conjugated with a CgA antibody and a SERS reporter, was applied to detect CgA in adrenal tumors. Results: Our data reveal that the results of the CgA-SERS probe method were almost identical to those of western blot and superior to those of traditional immunohistochemistry. Conclusion: This study offers a novel strategy to detect CgA in adrenal tumors and provides more reliable protein test results than traditional immunohistochemistry analysis for adrenal pathologists, meaning that it might be a better clinical reference for the diagnosis of pheochromocytoma.