Electrochemical Detection of Dopamine Using Green Synthesized Gold Nanoparticles from<i>Strobilanthes Kunthiana’s</i>Leaf Extract
Gokul Sridharan, Ridhu Varshini Murugan, Raji Atchudan, Sandeep Arya, Ashok K. Sundramoorthy
Abstract
Dopamine (DA) is an important neurotransmitter and has been linked to several neurological diseases. Due to its biological significance, electrochemical detection of dopamine has become a hot topic of research. Herein, by using gold nanoparticles (Au NPs) made through green synthesis, we demonstrate a unique method for the sensitive detection of DA. A Strobilanthes kunthiana leaves extract was used as a reducing and stabilizing agent during the green and sustainable synthesis of the Au NPs. Several characterizations including UV–Visible spectroscopy (absorption peak at 540 nm), Fourier-Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM) (average particle size of 31 nm), Energy Dispersive X-Ray Spectroscopy (EDAX), Elemental Mapping (E-MAP) and Electrochemical Impedance Spectroscopy (EIS) have been used to characterize the synthesized Au NPs. Moreover, electrochemical techniques like amperometry and Cyclic Voltammetry (CV) were used to confirm the stability and linearity of the Au NPs-based sensor for the determination of DA. The linear range of dopamine determination was found to be from 0.1 [Formula: see text]M to 356 [Formula: see text]M with the detection limit of 0.1 [Formula: see text]M.