A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
Yanqiong Chen, Huan Tao, Silan Shen, Zhiyong Miao, Lili Li, Yongqian Jia, Hu Zhang, Xiufeng Bai, Xin‐Yuan Fu
Abstract
The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (–)-Huperzine A worked well in changing the –1 ribosomal frameshifting of SARS-CoV-2 in vitro.
Topics & Concepts
Translational frameshiftFrameshift mutationComputational biologyVirologyOpen reading frameSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)BiologyCoronavirus disease 2019 (COVID-19)GeneticsMedicineMutationGenePeptide sequenceDiseaseInfectious disease (medical specialty)PathologyRNA and protein synthesis mechanismsAntimicrobial Peptides and ActivitiesCancer therapeutics and mechanisms