Phytochemical profiling, antidiabetic, antitumoral and cytotoxic potential of <i>Psidium cattleianum</i> Afzel. ex Sabine leaves of red variety
Yasmine de Melo Barradas, Felipe Tecchio Borsoi, Marina Volpato Dacoreggio, Liziane Schittler Moroni, Beatriz da Silva Rosa Bonadiman, Filomena Marafon, Clevison Luiz Giacobbo, Margarete Dulce Bagatini, Aniela Pinto Kempka
Abstract
In this study, phytochemical profiling, and antidiabetic, antitumoral and cytotoxic potential of aqueous extracts of leaves of red variety of Psidium cattleianum Afzel. ex Sabine were investigated. The extracts were obtained using a cellulase complex. The total phenolic compounds (TPC) were determined, and the individual phenolic compounds were quantified by HPLC-ESI-MS/MS. For the TPC, the amounts varied from 85.91 to 106.33 mg EAG g−1. Eighteen compounds have been identified. The compounds with the highest concentrations were gallic acid, quercetin and protocatechuic acid. Antidiabetic activity was obtained through α-amylase and α-glucosidase inhibition tests. The extract inhibited 17.94% of α-amylase activity and 73.34% of α-glucosidase activity. The antitumoral activity in cells of cutaneous melanoma (SK-MEL-28) and the cytotoxic activity was determined in peripheral blood mononuclear cells (PBMC). The cellular migration was determined for cells SK-MEL-28. Antitumoral effects on cells SK-MEL-28 were observed and the absence of cytotoxicity on the PBMCs.