Improving the Sensitivity for Quantifying Heparan Sulfate from Biological Samples
Zhangjie Wang, Vijay M. Dhurandhare, Cressida Mahung, Katelyn Arnold, Jine Li, Guowei Su, Ding Xu, Robert Maile, Jian Liu
Abstract
C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide. We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model. The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.
Topics & Concepts
ChemistryHeparan sulfateDisaccharideIn vivoSyndecan 1SulfationBiological fluidsBiochemistryHeparinCellChromatographyBiologyBiotechnologyProteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins ResearchNeuropeptides and Animal Physiology