In Situ Identification of Secondary Structures in Unpurified <i>Bombyx mori</i> Silk Fibrils Using Polarized Two-Dimensional Infrared Spectroscopy
Giulia Giubertoni, Federico Caporaletti, Steven J. Roeters, Adam S. Chatterley, Tobias Weidner, Peter R. Laity, Chris Holland, Sander Woutersen
Abstract
silk feedstock. Without any additional peak fitting, we find that the initial effect of hydration is an increase of the random coil content at the expense of the helical content, while the β-sheet content is unchanged and only increases at a later stage. This paper demonstrates that 2D-IR can be a valuable tool for characterizing biomaterials.
Topics & Concepts
Bombyx moriSILKRandom coilInfrared spectroscopyProtein secondary structureInfraredSpectroscopyIn situChemistryFibrilAmideMaterials scienceCharacterization (materials science)Analytical Chemistry (journal)CrystallographyNanotechnologyComposite materialOpticsOrganic chemistryBiochemistryPhysicsQuantum mechanicsGeneSilk-based biomaterials and applicationsInsect and Arachnid Ecology and BehaviorInsect Utilization and Effects