Two-dimensional FeCu-metal-organic-framework boosts peroxidase activity for antibacterial application
Lu YiXuan, Yaru Guo, Xiaohua Guo, Shihan Zhang, F. Liu, Shuai Ding, Dandan Wang, Yanpei Wang, Kongliang Li, Yuchen Wang, Youxing Liu, Xuliang Deng
Abstract
The overuse of antibiotics has led to the emergence of drug-resistant bacteria, posing a serious threat to human health and increasing the medical burden. Metal–organic frameworks (MOFs) have shown great potential as enzyme mimics for antibacterial applications; however, their performance is often limited by the low density and poor accessibility of active sites. Herein, we report an innovative surface-active agent intercalation–assisted confinement growth strategy for constructing two-dimensional (2D) NH₂-BDC-Cu, Fe nanosheets (NSs) with highly exposed active site to enhance reactive oxygen species (ROS) generation, thereby improving the antibacterial efficiency against drug-resistant bacteria. We demonstrate that the synthesized 2D NH₂-BDC-Cu, Fe NSs exhibit 6.6-fold higher ROS production compared to NH₂-BDC-Cu, Fe nanopowders (NPs). Consequently, NH₂-BDC-Cu, Fe NSs achieve 100 % antibacterial activity against both Staphylococcus aureus and Escherichia coli within 1 h, markedly outperforming NH₂-BDC-Cu, Fe NPs and previously reported biomaterials. Moreover, in vivo experiments confirm that NH₂-BDC-Cu, Fe NSs accelerate wound healing, achieving a 100 % healing rate within 10 days, which is substantially higher than that of NH₂-BDC-Cu, Fe NPs. Overall, this work introduces a novel and efficient strategy for fabricating 2D MOF nanoenzymes with high active-site exposure, offering great promise for the development of advanced biomaterial-based antibacterial therapies.