ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms
Jason C. Casler, Allison L. Zajac, Fernando M. Valbuena, Daniela Sparvoli, Okunola Jeyifous, Aaron P. Turkewitz, Sally Horne‐Badovinac, William N. Green, Benjamin S. Glick
Abstract
Exocytic membrane traffic can be visualized using regulatable fluorescent secretory cargoes. In cells from diverse eukaryotes, a red fluorescent fusion protein termed ESCargo can be trapped as aggregates in the endoplasmic reticulum and then released by ligand addition to create a wave of transport through the secretory pathway. This tool should have broad utility.
Topics & Concepts
Endoplasmic reticulumBiologySecretory pathwayCell biologySecretory proteinFluorescenceFusion proteinGreen fluorescent proteinTransport proteinSecretionBiochemistryGolgi apparatusRecombinant DNAGeneQuantum mechanicsPhysicsCellular transport and secretionPhotosynthetic Processes and MechanismsMicrotubule and mitosis dynamics