Protocols for endothelial cell isolation from mouse tissues: kidney, spleen, and testis
Sébastien J. Dumas, Elda Meta, Nadine V. Conchinha, Liliana Sokol, Rongyuan Chen, Mila Borri, Laure-Anne Teuwen, Koen Veys, Melissa García‐Caballero, Vincent Geldhof, Lucas Treps, Pauline de Zeeuw, Kim D. Falkenberg, Charlotte Dubois, Magdalena Parys, Laura de Rooij, Kateřina Rohlenová, Jermaine Goveia, Luc Schoonjans, Mieke Dewerchin, Guy Eelen, Xuri Li, Joanna Kalucka, Peter Carmeliet
Abstract
Endothelial cells (ECs) exhibit phenotypic and functional tissue specificities, critical for studies in the vascular field and beyond. Thus, tissue-specific methods for isolation of highly purified ECs are necessary. Kidney, spleen, and testis ECs are relevant players in health and diseases such as chronic kidney disease, acute kidney injury, myelofibrosis, and cancer. Here, we provide tailored protocols for rapid and reproducible EC purification established for scRNA sequencing from these adult murine tissues using the combination of magnetic- and fluorescence-activated cell sorting. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020) and Dumas et al. (2020).