Mitochondria-targeted fluorescent probe with long wavelength emission for detecting H2S and its application in foodstuff, water and living cells
Ling Xie, Renjie Wang, Congbin Fan, Yayi Tu, Gang Liu, Shouzhi Pu
Abstract
Hydrogen sulfide (H 2 S) is crucial to cellular energy production, apoptosis, and redox homeostasis in mitochondria of living cells. In this work, a unique mitochondria-targeting fluorescence probe ( DDMI ) was established for H 2 S determination based on styrylpyridinium scaffold. When DDMI was treated with H 2 S, it showed significant fluorescence enhancement at 623 nm, with good selectivity, and high sensitivity. In addition, the “turn-on” fluorescent probe DDMI could detect H 2 S in water samples with good recoveries in the range of 95.4 %–105.6 % and track the degree of food spoilage by visualizing the change of DDMI -loaded test strips. Furthermore, the established probe DDMI was successfully used for monitoring exogenous H 2 S in living cells and mitochondria targeting. These results paved the way for success in developing a technology that could be used to identify H 2 S in environment, foodstuff, and living cells.