Dipeptidyl peptidase‐IV inhibitory potential of alpha‐lactalbumin extracted from milk of <i>Gir</i> cows: A <i>Bos indicus</i> species
Ashok Kumar, M. H. Sathish Kumar, C. S. Rajani, Latha Sabikhi, Laxmana Naik N
Abstract
An attempt has been made to assess the dipeptidyl peptidase‐IV (DPP‐IV) inhibitory potential of α‐lactalbumin separated from milk of Gir cow. α‐lactalbumin hydrolysates were prepared using three different enzymes at different enzyme‐to‐substrate (E:S) ratios (1:25, 1:50 and 1:100) treated for 2‐ to 12‐h durations. Pepsin‐treated hydrolysates showed maximum DPP‐IV inhibition of 87.81 ± 0.84% (IC 50 = 0.78 mg/mL). The permeate of 3 kDa, which exhibited 84.47 ± 2.29% DPP‐IV inhibition, was fractionated through RP‐HPLC. All fractions were analysed for DPP‐IV inhibition activity. One novel peptide was identified through LC‐MS/MS, which showed adequate DPP‐IV inhibition (IC 50 = 4.93 mM).